Change search
ReferencesLink to record
Permanent link

Direct link
Serum sFAS levels are elevated in ANCA-positive vasculitis compared with other autoimmune diseases
Department of Clinical Immunology, Karolinska Institutet, Huddinge.
Department of Renal Medicine, Karolinska Institutet, Huddinge University.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Immunology.
Department of Clinical Immunology, Karolinska Institutet, Huddinge.
Show others and affiliations
2002 (English)In: Journal of Clinical Immunology, ISSN 0271-9142, E-ISSN 1573-2592, Vol. 22, no 4, 220-227 p.Article in journal (Refereed) Published
Abstract [en]

The role of the Fas/FasL system in ANCA-associated vasculitis is unclear. We therefore assessed levels of soluble Fas (sFas) in sera and Fas expression on mononuclear cells from patients with ANCA-positive vasculitis and compared the results with those found in other rheumatic diseases. Serum levels of sFas were determined by ELISA. The ANCA-positive vasculitis patients studied included 29 at onset, 17 in first remission while on therapy, and 12 in quiescence. For comparison, 10 patients with Sjogren’s syndrome (SS), 14 patients with systemic lupus erythematosus (SLE), 29 patients with rheumatoid arthritis (RA), 7 patients on dialysis (DP), and 26 healthy controls (HC) were studied. In addition, Fas expression in mononuclear cells was examined at the mRNA level using reverse transcriptase (RT)-PCR in 6 vasculitis patients at onset and in first remission. The expression of CD95 on the surface of leukocytes was determined by flow cytometry in 6 vasculitis patients at onset of the disease, in 6 patients in clinical remission, and in 6 HC. Expression of Fas and FasL in renal biopsy specimens was studied using immunohistochemistry. Patients with vasculitis had high sFas levels irrespective of disease phase. Both vasculitis patients and patients with RA and SLE had significantly increased sFas levels compared with healthy controls. All patient groups had sFas levels, which correlated with raised serum creatinine values. However, the sFas levels in vasculitis patients in first remission and in quiescence were increased despite a lower serum creatinine compared with onset. Some of the vasculitis patients showed an increased mRNA expression of Fas in mononuclear cells after treatment, suggesting that Fas production fluctuates with the intensity of the disease. The expression of CD95 on leukocytes was slightly decreased in vasculitis patients compared to healthy controls. No alterations of Fas and FasL expression were seen in renal biopsy specimens. These results show that ANCA-positive vasculitis patients have high sFas levels and that the levels remain elevated even in clinical remission. The findings indicate that perturbations in the Fas/Fas ligand system may play a role in the disease process in ANCA vasculitis.

Place, publisher, year, edition, pages
2002. Vol. 22, no 4, 220-227 p.
Keyword [en]
Fas; vasculitis; therapy
National Category
Rheumatology and Autoimmunity
URN: urn:nbn:se:umu:diva-39162DOI: 10.1023/A:1016040925295OAI: diva2:388922
Available from: 2011-01-18 Created: 2011-01-18 Last updated: 2011-01-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Eneslätt, KjellRantapää-Dahlqvist, Solbritt
By organisation
Clinical ImmunologyReumatology
In the same journal
Journal of Clinical Immunology
Rheumatology and Autoimmunity

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 33 hits
ReferencesLink to record
Permanent link

Direct link