The effects of storage time and sampling season on the stability of serum 25-hydroxy vitamin D and androstenedione
2010 (English)In: Nutrition and Cancer, ISSN 0163-5581, E-ISSN 1532-7914, Vol. 62, no 1, 51-57 p.Article in journal (Refereed) Published
Knowledge of the stability of serum samples stored in large biobanks is pivotal for reliable assessment of hormone-dependent disease risks. We studied the effects of sample storage time and season of serum sampling on the stability of 25-hydroxy vitamin D (25-OHD) and androstenedione in a stratified random sample of 402 women, using paired sera from the Finnish Maternity Cohort. Serum samples selected were donated between 6 and 24 yr ago. The storage time did not affect serum 25-OHD and androstenedione levels. However, there was a significant mean difference in the 25-OHD levels of sera withdrawn during winter (first sample) vs. during summer (second sample; -18.4 nmol/l, P ≤ 0.001). Also at the individual level, there were significant differences in average 25-OHD levels between individuals with the paired sera taken at winter–winter compared with other alternatives (summer–winter, winter–summer, and summer–summer). The androstenedione levels showed no such differences. Long-term storage does not affect serum 25-OHD and androstenedione levels, but sampling season is an important determinant of 25-OHD levels. Stored serum samples can be used to study disease associations with both hormones. However, sampling season needs to be taken into account for 25-OHD by considering matching and stratification and, if possible, serial sampling.
Place, publisher, year, edition, pages
2010. Vol. 62, no 1, 51-57 p.
hormone levels; prostate-cancer; testosterone; men; androgen; women; boston; growth; risk; d-3
Nutrition and Dietetics
IdentifiersURN: urn:nbn:se:umu:diva-39295DOI: 10.1080/01635580903191460ISI: 000273197400005PubMedID: 20043259OAI: oai:DiVA.org:umu-39295DiVA: diva2:390329