Structure of the sortase AcSrtC-1 from Actinomyces oris
2011 (English)In: Acta Crystallographica Section D: Biological Crystallography, ISSN 0907-4449, E-ISSN 1399-0047, Vol. 67, 212-217 p.Article in journal (Refereed) Published
The crystal structure of the sortase AcSrtC-1 from the oral microorganism Actinomyces oris has been determined to 2.4 Å resolution. AcSrtC-1 is a cysteine transpeptidase that is responsible for the formation of fimbriae by the polymerization of a shaft protein. Similar to other pili-associated sortases, the AcSrtC-1 active site is protected by a flexible lid. The asymmetric unit contains five AcSrtC-1 molecules and their catalytic Cys-His-Arg triads are trapped in two different conformations. It is also shown that the thermostability of the enzyme is increased by the presence of calcium.
Place, publisher, year, edition, pages
International Union of Crystallography , 2011. Vol. 67, 212-217 p.
sortase, oral biofilm, fimbriae
Dentistry Dentistry Microbiology in the medical area
Research subject Molecular Biology; Odontology
IdentifiersURN: urn:nbn:se:umu:diva-40184DOI: 10.1107/S0907444911004215OAI: oai:DiVA.org:umu-40184DiVA: diva2:398150
FunderSwedish Research Council