Change search
ReferencesLink to record
Permanent link

Direct link
SorLA regulates the activity of lipoprotein lipase by intracellular trafficking
Show others and affiliations
2011 (English)In: Journal of Cell Science, ISSN 0021-9533, E-ISSN 1477-9137, Vol. 124, 1095-1105 p.Article in journal (Refereed) Published
Abstract [en]

Many different tissues and cell types exhibit regulated secretion of lipoprotein lipase (LPL). However, the sorting of LPL in the trans Golgi network has not, hitherto, been understood in detail. Here, we characterize the role of SorLA (officially known as SorLA-1 or sortilin-related receptor) in the intracellular trafficking of LPL. We found that LPL bound to SorLA under neutral and acidic conditions, and in cells this binding mainly occurred in vesicular structures. SorLA expression changed the subcellular distribution of LPL so it became more concentrated in endosomes. From the endosomes, LPL was further routed to the lysosomes, which resulted in a degradation of newly synthesized LPL. Consequently, an 80% reduction of LPL activity was observed in cells that expressed SorLA. By analogy, SorLA regulated the vesicle-like localization of LPL in primary neuronal cells. Thus, LPL binds to SorLA in the biosynthetic pathway and is subsequently transported to endosomes. As a result of this SorLA mediated-transport, newly synthesized LPL can be routed into specialized vesicles and eventually sent to degradation, and its activity thereby regulated.

Place, publisher, year, edition, pages
2011. Vol. 124, 1095-1105 p.
Keyword [en]
Intracellular trafficking, Lipoprotein lipase, SorLA (SORL1), Vps10p-domain receptor
National Category
Medical and Health Sciences
Research subject
Physical Chemistry
URN: urn:nbn:se:umu:diva-40820DOI: 10.1242/jcs.072538PubMedID: 21385844OAI: diva2:403026
Available from: 2011-03-10 Created: 2011-03-10 Last updated: 2011-10-31Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Nilsson, Stefan KOlivecrona, Gunilla
By organisation
Physiological chemistry
In the same journal
Journal of Cell Science
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 84 hits
ReferencesLink to record
Permanent link

Direct link