E2F activation of S phase promoters via association with HCF-1 and the MLL family of histone H3K4 methyltransferases.
2007 (English)In: Molecular Cell, ISSN 1097-2765, E-ISSN 1097-4164, Vol. 27, no 1, 107-119 p.Article in journal (Refereed) Published
E2F transcriptional regulators control human-cell proliferation by repressing and activating the transcription of genes required for cell-cycle progression, particularly the S phase. E2F proteins repress transcription in association with retinoblastoma pocket proteins, but less is known about how they activate transcription. Here, we show that the human G1 phase regulator HCF-1 associates with both activator (E2F1 and E2F3a) and repressor (E2F4) E2F proteins, properties that are conserved in insect cells. Human HCF-1-E2F interactions are versatile: their associations and binding to E2F-responsive promoters are cell-cycle selective, and HCF-1 displays coactivator properties when bound to the E2F1 activator and corepressor properties when bound to the E2F4 repressor. During the G1-to-S phase transition, HCF-1 recruits the mixed-lineage leukemia (MLL) and Set-1 histone H3 lysine 4 methyltransferases to E2F-responsive promoters and induces histone methylation and transcriptional activation. These results suggest that HCF-1 induces cell-cycle-specific transcriptional activation by E2F proteins to promote cell proliferation.
Place, publisher, year, edition, pages
Elsevier (Cell Press) , 2007. Vol. 27, no 1, 107-119 p.
IdentifiersURN: urn:nbn:se:umu:diva-41105DOI: 10.1016/j.molcel.2007.05.030PubMedID: 17612494OAI: oai:DiVA.org:umu-41105DiVA: diva2:404662