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Cryo-EM reveals promoter DNA binding and conformational flexibility of the general transcription factor TFIID
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2009 (English)In: Structure, ISSN 0969-2126, E-ISSN 1878-4186, Vol. 17, no 11, 1442-1452 p.Article in journal (Refereed) Published
Abstract [en]

The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the TFIID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.

Place, publisher, year, edition, pages
2009. Vol. 17, no 11, 1442-1452 p.
Keyword [en]
proteins; DNA
URN: urn:nbn:se:umu:diva-41108DOI: 10.1016/j.str.2009.09.007PubMedID: 19913479OAI: diva2:404693
Available from: 2011-03-18 Created: 2011-03-18 Last updated: 2012-08-10Bibliographically approved

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Rofougaran, RezaHofer, Anders
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Department of Medical Biochemistry and Biophysics
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