1H HRMAS NMR Derived Bio-markers Related to Tumor Grade, Tumor Cell Fraction, and Cell Proliferation in Prostate Tissue Samples
2011 (English)In: Biomarker Insights, ISSN 1177-2719, Vol. 6, 39-47 p.Article in journal (Refereed) Published
A high-resolution magic angle spinning NMR spectroscopic approach is presented for evaluating the occurrence, amount and aggressiveness of cancer in human prostate tissue samples. Using this technique, key metabolites in malignant and non-malignant samples (n = 149) were identified, and patterns of their relative abundance were analyzed by multivariate statistical methods. Ratios of various metabolites – including (glycerophophorylcholine + phosphorylcholine)/creatine, myo-inositol/scyllo-inositol, scyllo-inositol/creatine, choline/creatine, and citrate/creatine – correlated with: i) for non-malignant tissue samples, the distance to the nearest tumor and its Gleason score and; ii) the fraction of tumor cells present in the sample; and iii) tumor cell proliferation (Ki67 labelling index). This NMR-based approach allows the extraction of information that could be useful for developing novel diagnostic methods for prostate cancer.
Place, publisher, year, edition, pages
Libertas Academica Ltd , 2011. Vol. 6, 39-47 p.
prostate cancer, HRMAS, MRSI, inositol, Gleason score, Ki67
Cancer and Oncology
IdentifiersURN: urn:nbn:se:umu:diva-41205DOI: 10.4137/BMI.S6794OAI: oai:DiVA.org:umu-41205DiVA: diva2:405031