Change search
ReferencesLink to record
Permanent link

Direct link
Regulation by SIRPα of dendritic cell homeostasis in lymphoid tissues
Gunma University, Gunma University Graduate School of Medicine.
Gunma University.
Gunma University.
Gunma University.
Show others and affiliations
2010 (English)In: Blood, ISSN 0006-4971, E-ISSN 1528-0020, Vol. 116, no 18, 3517-3525 p.Article in journal (Refereed) Published
Abstract [en]

The molecular basis for regulation of dendritic cell (DC) development and homeostasis remains unclear. Signal regulatory protein α (SIRPα), an immunoglobulin superfamily protein that is predominantly expressed in DCs, mediates cell-cell signaling by interacting with CD47, another immunoglobulin superfamily protein. We now show that the number of CD11c(high) DCs (conventional DCs, or cDCs), in particular, that of CD8-CD4+ (CD4+) cDCs, is selectively reduced in secondary lymphoid tissues of mice expressing a mutant form of SIRPα that lacks the cytoplasmic region. We also found that SIRPα is required intrinsically within cDCs or DC precursors for the homeostasis of splenic CD4+ cDCs. Differentiation of bone marrow cells from SIRPα mutant mice into DCs induced by either macrophage-granulocyte colony-stimulating factor or Flt3 ligand in vitro was not impaired. Although the accumulation of the immediate precursors of cDCs in the spleen was also not impaired, the half-life of newly generated splenic CD4+ cDCs was markedly reduced in SIRPα mutant mice. Both hematopoietic and nonhematopoietic CD47 was found to be required for the homeostasis of CD4+ cDCs and CD8-CD4- (double negative) cDCs in the spleen. SIRPα as well as its ligand, CD47, are thus important for the homeostasis of CD4+ cDCs or double negative cDCs in lymphoid tissues.

Place, publisher, year, edition, pages
2010. Vol. 116, no 18, 3517-3525 p.
National Category
Basic Medicine
URN: urn:nbn:se:umu:diva-41354DOI: 10.1182/blood-2010-03-277244ISI: 000283853200021PubMedID: 20682853OAI: diva2:405686
Available from: 2011-03-23 Created: 2011-03-23 Last updated: 2013-10-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Oldenborg, Per-Arne
By organisation
Histology and Cell Biology
In the same journal
Basic Medicine

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 50 hits
ReferencesLink to record
Permanent link

Direct link