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Efficacy of olfactory ensheathing cells to support regeneration after spinal cord injury is influenced by method of culture preparation
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy. Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Hand Surgery.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
2011 (English)In: Experimental Neurology, ISSN 0014-4886, E-ISSN 1090-2430, Vol. 229, no 1, 132-142 p.Article in journal (Refereed) Published
Abstract [en]

Olfactory ensheathing cells (OEC) have been shown to stimulate regeneration, myelination and functional recovery in different spinal cord injury models. However, recent reports from several laboratories have challenged this treatment strategy. The discrepancy in results could be attributed to many factors including variations in culture protocols. The present study investigates whether the differences in culture preparation could influence neuroprotective and growth-promoting effects of OEC after transplantation into the injured spinal cord. Primary OEC cultures were purified using method of differential cell adhesion (a-OEC) or separated with immunomagnetic beads (b-OEC). After cervical C4 hemisection in adult rats, short-term (3weeks) or long-term (7weeks) cultured OEC were transplanted into the lateral funiculus at 1mm rostral and caudal to the transection site. At 3-8weeks after transplantation, labeled OEC were mainly found in the injection sites and in the trauma zone. Short-term cultured a-OEC supported regrowth of rubrospinal, raphaespinal and CGRP-positive fibers, and attenuated retrograde degeneration in the red nucleus. Short-term cultured b-OEC failed to promote axonal regrowth but increased the density of rubrospinal axons within the dorsolateral funiculus and provided significant neuroprotection for axotomized rubrospinal neurons. In addition, short-term cultured OEC attenuated sprouting of rubrospinal terminals. In contrast, long-term cultured OEC neither enhanced axonal growth nor prevented retrograde cell death. The results suggest that the age of OEC in culture and the method of cell purification could affect the efficacy of OEC to support neuronal survival and regeneration after spinal cord injury.

Place, publisher, year, edition, pages
2011. Vol. 229, no 1, 132-142 p.
Keyword [en]
Adult rats; Red nucleus; Olfactory ensheathing glia; Spinal cord trauma; Transplantation
National Category
Neurosciences
Identifiers
URN: urn:nbn:se:umu:diva-41352DOI: 10.1016/j.expneurol.2010.09.021PubMedID: 20932826OAI: oai:DiVA.org:umu-41352DiVA: diva2:405689
Available from: 2011-03-23 Created: 2011-03-23 Last updated: 2012-04-19Bibliographically approved

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Novikova, Liudmila NWiberg, MikaelNovikov, Lev N

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