Bone marrow- and adipose-derived stem cells show expression of myelin mRNAs and proteins
2010 (English)In: Regenerative medicine, ISSN 1746-076X, Vol. 5, no 3, 403-410 p.Article in journal (Refereed) Published
Aims: PNS myelin is formed by Schwann cells (SCs). In this study, we applied an in vitro model to study myelin formation, using bone marrow mesenchymal stem cells and adipose-derived stem cells differentiated into SC-like cells and co-cultured with dissociated adult dorsal root ganglia neurons.
Methods: Immunocytochemistry, reverse transcription-PCR and western blotting techniques were used to investigate the expression of myelin proteins at both the transcriptional and translational level.
Results: Transcripts for protein zero, peripheral myelin protein 22 and myelin basic protein were detected in differentiated stem cells following co-culture with neuronal cells. Furthermore, protein zero, peripheral myelin protein 22 and myelin basic proteins were recognized in the co-cultures. These results were consistent with immunostaining of myelin proteins and with observation by electron microscopy.
Conclusion: Both types of adult stems cells differentiated into SC-like cells have potential to myelinate neuronal cells during regeneration, being functionally identical to SCs of the PNS.
Place, publisher, year, edition, pages
2010. Vol. 5, no 3, 403-410 p.
adipose, mesenchymal, myelin, stem cells
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:umu:diva-41349DOI: 10.2217/rme.10.15PubMedID: 20455651OAI: oai:DiVA.org:umu-41349DiVA: diva2:405697