Change search
ReferencesLink to record
Permanent link

Direct link
Regeneration potential and survival of transplanted undifferentiated adipose tissue-derived stem cells in peripheral nerve conduits
University of Manchester, University Hospital of Basel.
University of Manchester.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
University Hospital of Basel.
Show others and affiliations
2010 (English)In: Journal of plastic, reconstructive & aesthetic surgery : JPRAS, ISSN 1878-0539, Vol. 63, no 12, e811-e817 p.Article in journal (Refereed) Published
Abstract [en]

Adipose tissue-derived stem cells (ADSCs) have shown potential for the treatment of nerve injuries. Most previous efforts have aimed at stimulating regeneration by using neural-differentiation protocols, but the potential of undifferentiated ADSCs to enhance axonal growth as well as their ability to transdifferentiate in situ have been poorly investigated. In this study, using a rat sciatic nerve model we show that ADSCs, transplanted in an artificial nerve conduit, stimulate axonal outgrowth from the proximal nerve stump and evoke greater Schwann cell (SC) proliferation/intrusion in the distal stump. To track the fate of the transplanted cells, we used green fluorescent protein (GFP)-labelling and polymerase chain reaction (PCR) for the detection of the sex determining region Y (SRY) gene in the donor male cells. Both methods indicated a lack of significant quantities of viable cells 14 days after transplantation. These results suggest that any regenerative effect of transplanted ADSCs is more likely to be mediated by an initial boost of released growth factors and/or by an indirect effect on endogenous SCs activity. Future studies need to address long-term cell survival in tissue-engineered nerve conduits to improve the neuroregenerative potential of ADSCs.

Place, publisher, year, edition, pages
2010. Vol. 63, no 12, e811-e817 p.
Keyword [en]
Adult Stem Cells; Peripheral Nerve; Regeneration; Schwann cell; Adipose tissue
National Category
URN: urn:nbn:se:umu:diva-41344DOI: 10.1016/j.bjps.2010.08.013ISI: 000284463500002PubMedID: 20851070OAI: diva2:405703
Available from: 2011-03-23 Created: 2011-03-23 Last updated: 2016-02-22Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Kalbermatten, Daniel FKingham, Paul J
By organisation

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 44 hits
ReferencesLink to record
Permanent link

Direct link