Modifying the cellular transport of DNA-based vaccines alters the immune response to hantavirus nucleocapsid protein
2001 (English)In: Vaccine, ISSN 0264-410X, E-ISSN 1873-2518, Vol. 19, no 28-29, 3820-3829 p.Article in journal (Refereed) Published
Puumala virus is a member of the hantavirus genus (family Bunyaviridae) and is one of the causative agents of hemorrhagic fever with renal syndrome (HFRS) in Europe. A genetic vaccination approach was conducted to investigate if the immune response could be modulated using different cellular secretion and/or localisation signals, and the immune responses were analysed in BALB/c mice and in a bank vole infectious model. Rodents vaccinated with DNA constructs encoding the antigen fused to an amino-terminal secretion signal raised significantly higher antibody levels when compared to using constructs lacking secretion signals. Furthermore, the ratios of the IgG subclasses (IgG2a/IgG1) were raised by the use of cellular localisation signals, indicating a more pronounced Th1-type of immune response. The majority of the mice, or bank voles, immunised with DNA encoding a secreted form of the antigen showed a positive lymphoproliferative response and were protected against challenge with Puumala virus (strain Kazan-wt).
Place, publisher, year, edition, pages
2001. Vol. 19, no 28-29, 3820-3829 p.
Cellular transport; DNA vaccines; Hantavirus; Immune response; Puumala virus
IdentifiersURN: urn:nbn:se:umu:diva-42060DOI: 10.1016/S0264-410X(01)00151-7PubMedID: 11427253OAI: oai:DiVA.org:umu-42060DiVA: diva2:408451