Change search
ReferencesLink to record
Permanent link

Direct link
Pyrosequencing of antibiotic-contaminated river sediments reveals high levels of resistance and gene transfer elements
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Show others and affiliations
2011 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 6, no 2, e17038- p.Article in journal (Refereed) Published
Abstract [en]

The high and sometimes inappropriate use of antibiotics has accelerated the development of antibiotic resistance, creating a major challenge for the sustainable treatment of infections world-wide. Bacterial communities often respond to antibiotic selection pressure by acquiring resistance genes, i.e. mobile genetic elements that can be shared horizontally between species. Environmental microbial communities maintain diverse collections of resistance genes, which can be mobilized into pathogenic bacteria. Recently, exceptional environmental releases of antibiotics have been documented, but the effects on the promotion of resistance genes and the potential for horizontal gene transfer have yet received limited attention. In this study, we have used culture-independent shotgun metagenomics to investigate microbial communities in river sediments exposed to waste water from the production of antibiotics in India. Our analysis identified very high levels of several classes of resistance genes as well as elements for horizontal gene transfer, including integrons, transposons and plasmids. In addition, two abundant previously uncharacterized resistance plasmids were identified. The results suggest that antibiotic contamination plays a role in the promotion of resistance genes and their mobilization from environmental microbes to other species and eventually to human pathogens. The entire life-cycle of antibiotic substances, both before, under and after usage, should therefore be considered to fully evaluate their role in the promotion of resistance.

Place, publisher, year, edition, pages
2011. Vol. 6, no 2, e17038- p.
Keyword [en]
mediated quinolone resistance; aquatic environment; bacteria; soil; diversity; integrons; database; water; identification; metagenomics
National Category
Biological Sciences
URN: urn:nbn:se:umu:diva-42236DOI: 10.1371/journal.pone.0017038PubMedID: 21359229OAI: diva2:408974
Available from: 2011-04-06 Created: 2011-04-06 Last updated: 2012-05-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Fick, JerkerGrabic, RomanSöderström, Hanna
By organisation
Department of Chemistry
In the same journal
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 159 hits
ReferencesLink to record
Permanent link

Direct link