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Differential control of spontaneous and evoked GABA release by presynaptic L-type Ca(2+) channels in the rat medial preoptic nucleus
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology.
2010 (English)In: Journal of Neurophysiology, ISSN 0022-3077, E-ISSN 1522-1598, Vol. 104, no 1, 200-209 p.Article in journal (Refereed) Published
Abstract [en]

To clarify the role of presynaptic L-type Ca(2+) channels in GABA-mediated transmission in the medial preoptic nucleus (MPN), spontaneous, miniature, and impulse-evoked inhibitory postsynaptic currents (sIPSCs, mIPSCs, and eIPSCs, respectively) were recorded from MPN neurons in a slice preparation from rat brain. The effects of different stimulus protocols and pharmacological tools to detect contributions of L-type Ca(2+) channels and of Ca(2+)-activated K(+) (K(Ca)) channels were analyzed. Block of L-type channels did not affect the sIPSC and mIPSC properties (frequency, amplitude, decay time course) in the absence of external stimulation but unexpectedly potentiated the eIPSCs evoked at low stimulus frequency (0.1-2.0 Hz). This effect was similar to and overlapping with the effect of K(Ca)-channel blockers. High-frequency stimulation (50 Hz for 10 s) induced a substantial posttetanic potentiation (PTP) of the eIPSC amplitude and of the sIPSC frequency. Block of L-type channels still potentiated the eIPSC during PTP, but in contrast, reduced the sIPSC frequency during PTP. It was concluded that L-type channels provide a means for differential control of spontaneous and impulse-evoked GABA release and that this differential control is prominent during short-term synaptic plasticity. Functional coupling of the presynaptic L-type channels to K(Ca) channels explains the observed effects on eIPSCs.

Place, publisher, year, edition, pages
2010. Vol. 104, no 1, 200-209 p.
National Category
Neurosciences
Identifiers
URN: urn:nbn:se:umu:diva-42241DOI: 10.1152/jn.00570.2009ISI: 000279586400018PubMedID: 20463198OAI: oai:DiVA.org:umu-42241DiVA: diva2:409015
Available from: 2011-04-06 Created: 2011-04-06 Last updated: 2017-12-11Bibliographically approved

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Malinina, EvgenyaDruzin, MichaelJohansson, Staffan

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