Ribosylurea accumulates in yeast urc4 mutants
2010 (English)In: Nucleosides, Nucleotides & Nucleic Acids, ISSN 1525-7770, E-ISSN 1532-2335, Vol. 29, no 4-6, 433-437 p.Article in journal (Refereed) Published
Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.
Place, publisher, year, edition, pages
2010. Vol. 29, no 4-6, 433-437 p.
Uracil degradation, ribosylurea, URC pathway, Saccharomyces (Lachancea) kluyveri
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-42558DOI: 10.1080/15257771003741265ISI: 000278713200029PubMedID: 20544532OAI: oai:DiVA.org:umu-42558DiVA: diva2:409655