Environmental policy integration, EPI, refers to the integration of environmental aspects and policy objectivesinto non‐environmental sector policies. EPI can be viewed as an environmental learning process, but whathappens when EPI is to travel across political levels? This article explores EPI in a previously neglected policyarena; the sub‐national level, where Biofuel Region (BFR) in the north Swedish counties Västerbotten andVästernorrland is studied in depth. According to previous studies, an environmental perspective is integratedinto bioenergy policy on the EU level and Swedish level, but is an environmental perspective present also onthe sub‐national level, within BFR? Furthermore, can the development in BFR be explained by cross‐levellearning? In a discussion of the impact of EU and Swedish bioenergy policy on the sub‐national bioenergydevelopment, the concluding discussion revolves around the overarching question of this study: what are thesub‐national implications of higher‐level EPI in a policy area?