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Expression and purification of active recombinant equine lysozyme in Escherichia coli
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2009 (English)In: Protein Engineering Design & Selection, ISSN 1741-0126, E-ISSN 1741-0134, Vol. 22, no 11, 649-654 p.Article in journal (Refereed) Published
Abstract [en]

Equine lysozyme (EL) is a calcium (Ca)-binding lysozyme and is an intermediary link between non-Ca-binding C-type lysozyme and alpha-lactalbumin. The feature of lysozymes to assemble into the fibrils has recently gained considerable attention for the investigation of the functional properties of these proteins. To study the structural and functional properties of EL, a synthetic gene was cloned and EL was overexpressed in Escherichia coli as a fused protein. The His-tagged recombinant EL was accumulated as inclusion bodies. Up to 50 mg/l of the recombinant EL could be achieved after purification by Ni(2+) affinity chromatography, refolding in the presence of arginine, CM-Sepharose column purification following TEV protease cleavage. The purified protein was functionally active, as determined by the lysozyme activity, proving the proper folding of protein. The purified lysozyme was used for the oligomerisation studies. The protein formed amyloid fibrils during incubation in acidic pH and elevated temperature. The recombinant EL forms two types of fibrils: ring shaped and linear, similar to the native EL.

Place, publisher, year, edition, pages
2009. Vol. 22, no 11, 649-654 p.
Keyword [en]
amyloid fibrils, equine lysozyme, heterologous expression, inclusion body
Identifiers
URN: urn:nbn:se:umu:diva-42966DOI: 10.1093/protein/gzp048PubMedID: 19651623OAI: oai:DiVA.org:umu-42966DiVA: diva2:410833
Available from: 2011-04-15 Created: 2011-04-15 Last updated: 2017-12-11Bibliographically approved

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Morozova-Roche, Ludmilla A
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Department of Medical Biochemistry and Biophysics
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