Change search
ReferencesLink to record
Permanent link

Direct link
Cation binding effects on the pH, thermal and urea denaturation transitions in alpha-lactalbumin.
Institute of Biological Physics, Pushchino, Russia.
1985 (English)In: Biophysical Chemistry, ISSN 0301-4622, E-ISSN 1873-4200, Vol. 21, no 1, 21-31 p.Article in journal (Refereed) Published
Abstract [en]

The binding of monovalent (Na+, K+) and divalent (Ca2+, Mg2+) cations to bovine alpha-lactalbumin at 20 and 37 degrees C has been studied by means of intrinsic protein fluorescence. The values of apparent binding constants for these ions obtained at 37 degrees C are about one order of magnitude lower than those measured at 20 degrees C. Urea and alkali (pH greater than 10) induce unfolding transitions which involve stable partially unfolded intermediates for all metal ion-bound forms of alpha-lactalbumin. Heating induces similar partially unfolded states. Nevertheless, the partially unfolded states induced by heating, urea, alkaline or acidic treatments are somewhat different in their tryptophan residue environment properties. The results have been interpreted in terms of a simple scheme of equilibria between metal-free and metal-bound forms in their native, partially unfolded and unfolded states. The scheme provides an approach to the quantitative interpretation of any transition equilibrium shift induced by a low molecular mass species able to be bound by a protein.

Place, publisher, year, edition, pages
1985. Vol. 21, no 1, 21-31 p.
URN: urn:nbn:se:umu:diva-43023PubMedID: 3971025OAI: diva2:411138
Available from: 2011-04-15 Created: 2011-04-15 Last updated: 2011-04-18

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Morozova, L A
In the same journal
Biophysical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 20 hits
ReferencesLink to record
Permanent link

Direct link