Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis
2006 (English)In: Clinical Microbiology and Infection, ISSN 1198-743X, E-ISSN 1469-0691, Vol. 12, no 2, 131-136 p.Article in journal (Refereed) Published
Sensitive and rapid detection of infection with Toxoplasma gondii in transplanted immunocompromised patients is crucial for a good prognosis. Two DNA fragments are used currently for detecting T. gondii infection by PCR, i.e., the B1 gene and a 529-bp repeat element that exists in 200-300 copies/genome. This study investigated whether targeting the 529-bp repeat element gives better sensitivity and accuracy than can be obtained when targeting the B1 gene (35 copies) when concentrations of T. gondii DNA are low. The results demonstrated that detection of the 529-bp repeat element increased diagnostic sensitivity and accuracy. Addition of an internal amplification control did not affect the PCR performance and was useful in order to monitor PCR inhibition by non-specific DNA in the LightCycler instrument. The real-time PCR was used successfully in a clinical context to monitor parasitaemia in the blood of a transplant recipient suffering from toxoplasmosis.
Place, publisher, year, edition, pages
2006. Vol. 12, no 2, 131-136 p.
B1 gene; diagnosis; internal amplification control; real-time PCR; repeat element; toxoplasmosis
IdentifiersURN: urn:nbn:se:umu:diva-43033DOI: 10.1111/j.1469-0691.2005.01332.xPubMedID: 16441450OAI: oai:DiVA.org:umu-43033DiVA: diva2:411154