Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptake
2010 (English)In: Genes & Development, ISSN 0890-9369, E-ISSN 1549-5477, Vol. 24, no 14, 1491-1495 p.Article in journal (Refereed) Published
Inactivation of the yeast nonsense-mediated mRNA decay (NMD) pathway stabilizes nonsense mRNAs and promotes readthrough of premature translation termination codons. Although the latter phenotype is thought to reflect a direct role of NMD factors in translation termination, its mechanism is unknown. Here we show that the reduced termination efficiency of NMD-deficient cells is attributable to increased expression of the magnesium transporter Alr1p and the resulting effects of elevated Mg2+ levels on termination fidelity. Alr1p levels increase because an upstream ORF in ALR1 mRNA targets the transcript for NMD. Our results demonstrate that NMD, at least in yeast, controls Mg2+ homeostasis and, consequently, translational fidelity.
Place, publisher, year, edition, pages
2010. Vol. 24, no 14, 1491-1495 p.
NMD; translation termination; magnesium uptake; uORF
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-43188DOI: 10.1101/gad.1930710ISI: 000279941500006OAI: oai:DiVA.org:umu-43188DiVA: diva2:412346