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Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptake
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
University Massachusetts, School of Medicine, Dept Mol Genet & Microbiol, Worcester, MA .
2010 (English)In: Genes & Development, ISSN 0890-9369, E-ISSN 1549-5477, Vol. 24, no 14, 1491-1495 p.Article in journal (Refereed) Published
Abstract [en]

Inactivation of the yeast nonsense-mediated mRNA decay (NMD) pathway stabilizes nonsense mRNAs and promotes readthrough of premature translation termination codons. Although the latter phenotype is thought to reflect a direct role of NMD factors in translation termination, its mechanism is unknown. Here we show that the reduced termination efficiency of NMD-deficient cells is attributable to increased expression of the magnesium transporter Alr1p and the resulting effects of elevated Mg2+ levels on termination fidelity. Alr1p levels increase because an upstream ORF in ALR1 mRNA targets the transcript for NMD. Our results demonstrate that NMD, at least in yeast, controls Mg2+ homeostasis and, consequently, translational fidelity.

Place, publisher, year, edition, pages
2010. Vol. 24, no 14, 1491-1495 p.
Keyword [en]
NMD; translation termination; magnesium uptake; uORF
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-43188DOI: 10.1101/gad.1930710ISI: 000279941500006OAI: diva2:412346
Available from: 2011-04-22 Created: 2011-04-22 Last updated: 2011-10-11Bibliographically approved

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Johansson, Marcus JO
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