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Two groups of phenylalanine biosynthetic operon leader peptides genes: a high level of apparently incidental frameshifting in decoding Escherichia coli pheL
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). (Björk)
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2011 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 39, no 8, 3079-3092 p.Article in journal (Refereed) Published
Abstract [en]

The bacterial pheL gene encodes the leader peptide for the phenylalanine biosynthetic operon. Translation of pheL mRNA controls transcription attenuation and, consequently, expression of the downstream pheA gene. Fifty-three unique pheL genes have been identified in sequenced genomes of the gamma subdivision. There are two groups of pheL genes, both of which are short and contain a run(s) of phenylalanine codons at an internal position. One group is somewhat diverse and features different termination and 5’-flanking codons. The other group, mostly restricted to Enterobacteria and including Escherichia coli pheL, has a conserved nucleotide sequence that ends with UUC_CCC_UGA. When these three codons in E. coli pheL mRNA are in the ribosomal E-, P- and A-sites, there is an unusually high level, 15%, of +1 ribosomal frameshifting due to features of the nascent peptide sequence that include the penultimate phenylalanine. This level increases to 60% with a natural, heterologous, nascent peptide stimulator. Nevertheless, studies with different tRNA(Pro) mutants in Salmonella enterica suggest that frameshifting at the end of pheL does not influence expression of the downstream pheA. This finding of incidental, rather than utilized, frameshifting is cautionary for other studies of programmed frameshifting.

Place, publisher, year, edition, pages
2011. Vol. 39, no 8, 3079-3092 p.
Keyword [en]
translation termination; transfer-rna; salmonella-typhimurium; nascent-peptide; messenger-rna; attenuation regulation; coding gap; in-vivo; ribosome; expression
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:umu:diva-43186DOI: 10.1093/nar/gkq1272PubMedID: 21177642OAI: diva2:412350
Available from: 2011-04-22 Created: 2011-04-22 Last updated: 2011-10-05Bibliographically approved

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Näsvall, S JoakimBjörk, Glenn R
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