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Analyzing p-bodies and stress granules in Saccharomyces cerevisae
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
2010 (English)In: Guide to yeast genetics: functional genomics, proteomics, and other systems analysis / [ed] Jonathan Weissman, Christine Guthrie, Gerald R. Fink, San Diego: Elsevier, 2010, 2, Vol. 470, 619-640 p.Chapter in book (Refereed)
Abstract [en]

Eukaryotic cells contain at least two types of cytoplasmic RNA protein (RNP) granules that contain nontranslating mRNAs. One such RNP granule is a P-body, which contains translationally inactive mRNAs and proteins involved in mRNA degradation and translation repression. A second such RNP granule is a stress granule which also contains mRNAs, some RNA binding proteins and several translation initiation factors, suggesting these granules contain mRNAs stalled in translation initiation. In this chapter, we describe methods to analyze P-bodies and stress granules in Saccharomyces cerevisiae, including procedures to determine if a protein or mRNA can accumulate in either granule, if an environmental perturbation or mutation affects granule size and number, and granule quantification methods.

Place, publisher, year, edition, pages
San Diego: Elsevier, 2010, 2. Vol. 470, 619-640 p.
, Methods in Enzymology, ISSN 0076-6879 ; 470
Keyword [en]
cytoplasmic processing bodies, messenger-rna decay, translational repression, human-cells, gene-expression, global analysis, body formation, budding yeast, localization, stability
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-43179DOI: 10.1016/S0076-6879(10)70025-2ISI: 000275827900025ISBN: 978-0-12-375171-3ISBN: 978-0-12-375172-0OAI: diva2:412356
Available from: 2011-04-22 Created: 2011-04-22 Last updated: 2015-09-24Bibliographically approved

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Nissan, Tracy
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