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Decapping activators in Saccharomyces cerevisiae act by multiple mechanisms
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
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2010 (English)In: Molecular Cell, ISSN 1097-2765, E-ISSN 1097-4164, Vol. 39, no 5, 773-783 p.Article in journal (Refereed) Published
Abstract [en]

Eukaryotic mRNA degradation often occurs in a process whereby translation initiation is inhibited and the mRNA is targeted for decapping. In yeast cells, Pat1, Scd6, Edc3, and Dhh1 all function to promote decapping by an unknown mechanism(s). We demonstrate that purified Scd6 and a region of Pat1 directly repress translation in vitro by limiting the formation of a stable 48S preinitiation complex. Moreover, while Pat1, Edc3, Dhh1, and Scd6 all bind the decapping enzyme, only Pat1 and Edc3 enhance its activity. We also identify numerous direct interactions between Pat1, Dcp1, Dcp2, Dhh1, Scd6, Edc3, Xrn1, and the Lsm1-7 complex. These observations identify three classes of decapping activators that function to directly repress translation initiation and/or stimulate Dcp1/2. Moreover, Pat1 is identified as critical in mRNA decay by first inhibiting translation initiation, then serving as a scaffold to recruit components of the decapping complex, and finally activating Dcp2.

Place, publisher, year, edition, pages
2010. Vol. 39, no 5, 773-783 p.
Keyword [en]
cytoplasmic processing bodies; messenger-rna decay; sm-like proteins; caenorhabditis-elegans; crystal-structure; in-vivo; translation; complex; identification; helicase
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-43192DOI: 10.1016/j.molcel.2010.08.025ISI: 000281941000014OAI: diva2:412358
Available from: 2011-04-22 Created: 2011-04-22 Last updated: 2011-10-11Bibliographically approved

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Nissan, Tracy
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Department of Molecular Biology (Faculty of Medicine)
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