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Novel role for a bacterial nucleoid protein in translation of mRNAs with suboptimal ribosome-binding sites
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
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2010 (English)In: Genes & Development, ISSN 0890-9369, E-ISSN 1549-5477, Vol. 24, no 13, 1345-1350 p.Article in journal (Refereed) Published
Abstract [en]

In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor stimulating translation of the malT mRNA. In vitro studies showed that H-NS facilitates a repositioning of the 30S preinitiation complex on the malT mRNA. H-NS stimulation of translation depended on the AU-rich -35 to -40 region of the mRNA. Several additional examples were found demonstrating a novel function for H-NS in translation of genes with suboptimal ribosome-binding sequences.

Place, publisher, year, edition, pages
2010. Vol. 24, no 13, 1345-1350 p.
Keyword [en]
Bacterial nucleoid; gene expression; translation; ribosome-binding sites; 5 '-untranslated region
National Category
Medical Genetics
Identifiers
URN: urn:nbn:se:umu:diva-43193DOI: 10.1101/gad.576310ISI: 000279405000004OAI: oai:DiVA.org:umu-43193DiVA: diva2:412361
Available from: 2011-04-22 Created: 2011-04-22 Last updated: 2017-12-11Bibliographically approved

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Publisher's full texthttp://www.ncbi.nlm.nih.gov/pubmed/20595230

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Park, Hyun-SookÖstberg, YngveJohansson, JörgenUhlin, Bernt Eric
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