Change search
ReferencesLink to record
Permanent link

Direct link
Physiological and transcriptomic characterization of a fliA mutant of Pseudomonas putida KT2440
Show others and affiliations
2010 (English)In: Environmental Microbiology Reports, ISSN 1758-2229, Vol. 2, no 3, 373-380 p.Article in journal (Refereed) Published
Abstract [en]

Pseudomonas putida KT2440 encodes 23 alternative sigma factors. The fliA gene, which encodes sigma 28, is in a cluster with other genes involved in flagella biosynthesis and chemotaxis. Reverse transcriptase-PCR revealed that this cluster is comprised of four independent transcriptional units: flhAF, fleNfliA, cheYZA and cheBmotAB. We generated a nonpolar fliA mutant by homologous recombination and tested its motility, adhesion to biotic and abiotic surfaces, and responses to various stress conditions. The mutant strain was nonmotile and exhibited decreased capacity to bind to corn seeds, although its ability to colonize the rhizosphere of plants was unaffected. The mutant was also affected in binding to abiotic surfaces and its ability to form biofilms decreased by almost threefold. In the fliA mutant background expression of 25 genes was affected: two genes were upregulated and 23 genes were downregulated. In addition to a number of motility and chemotaxis genes, the fliA gene product is also necessary for the expression of some genes potentially involved in amino acid utilization or stress responses; however, we were unable to assign specific phenotypes linked to these genes since the fliA mutant used the same range of amino acids as the parental strain, and was as tolerant as the wild type to stress imposed by heat, antibiotics, NaCl, sodium dodecyl sulfate, H2O2 and benzoate. Based on the sequence alignment of promoters recognized by FliA and genome in silico analysis, we propose that P. putida sigma 28 recognizes a TCAAG-t-N-12-GCCGATA consensus sequence located between -34 and -8 and that this sequence is preferentially associated with an AT-rich upstream region.

Place, publisher, year, edition, pages
2010. Vol. 2, no 3, 373-380 p.
Keyword [en]
cleavage pathway operon; escherichia-coli; promoter recognition; mutational analysis; positive regulator; secretion system; dna microarray; rna-polymerase; flagellar; motility
National Category
Environmental Sciences
URN: urn:nbn:se:umu:diva-43194DOI: 10.1111/j.1758-2229.2009.00084.xISI: 000279404300003OAI: diva2:412375
ISI Document Delivery No.: 619BU Times Cited: 0 Cited Reference Count: 41 Juan Rodriguez-Herva, Jose Duque, Estrella Antonia Molina-Henares, Maria Navarro-Aviles, Gloria van Dillewijn, Pieter de la Torre, Jesus Molina-Henares, Antonio J. Sanchez-de la Campa, Ana Ann Ran, F. Segura, Ana Shingler, Victoria Ramos, Juan-Luis ERA-NET [GEN2006-27750-C5-5-E/SYS]; CONSOLIDER-INGENIO [CSD2007-00005]; Junta de Andalucia [CIV-3010] This study was supported by the following grants: ERA-NET (GEN2006-27750-C5-5-E/SYS), CONSOLIDER-INGENIO (CSD2007-00005) and CIV-3010 from Junta de Andalucia. We thank A. Hurtado for DNA sequencing, M.M. Fandila and C. Lorente for secretarial assistance, and K. Shashok and B. Pakuts for improving the use of English in the manuscript. Wiley-blackwell publishing, inc MaldenAvailable from: 2011-04-22 Created: 2011-04-22 Last updated: 2011-10-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Shingler, Victoria
By organisation
Department of Molecular Biology (Faculty of Science and Technology)
In the same journal
Environmental Microbiology Reports
Environmental Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 88 hits
ReferencesLink to record
Permanent link

Direct link