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Proteomics of extreme freezing tolerance in Siberian spruce (Picea obovata)
Department of Biology, Norwegian University of Science and Technology.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Department of Biology, Norwegian University of Science and Technology.
2010 (English)In: Journal of Proteomics, ISSN 1874-3919, Vol. 73, no 5, 965-975 p.Article in journal (Refereed) Published
Abstract [en]

Differential expression of proteins in needles of the extreme freeze tolerant conifer Picea obovata during September, October and November was analyzed using DIGE technology and multivariate analysis. More than 1200 spots were detected, and the abundance of 252 of these spots was significantly altered during the course of acclimation. The 252 spots were clustered into five distinct expression profiles. Among the protein spots showing differential expression, 43 were identified by MALDI-TOF/TOF and twelve of them matched proteins associated with various biotic and abiotic stress responses in other plants. Dehydrins, Hsp70s, AAA+ ATPases, lipocalin, cyclophilins, glycine-rich protein (GNP) and several reactive oxygen intermediate scavenging proteins showed increased accumulation levels from September to November. The expression profiles and putative role of the identified proteins during acclimation and freezing tolerance are discussed.

Place, publisher, year, edition, pages
Elsevier , 2010. Vol. 73, no 5, 965-975 p.
Keyword [en]
freezing tolerance, Picea obovata, DIGE, MALDI-TOF/TOF, OPLS-DA, dehydrins
URN: urn:nbn:se:umu:diva-43519DOI: 10.1016/j.jprot.2009.12.010ISI: 000276167900010OAI: diva2:414430
Available from: 2011-05-03 Created: 2011-05-03 Last updated: 2011-05-05Bibliographically approved
In thesis
1. Proteomics and metabolomics in biological and medical applications
Open this publication in new window or tab >>Proteomics and metabolomics in biological and medical applications
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Biological processes in living organisms consist of a vast number of different molecular networks and interactions, which are complex and often hidden from our understanding. This work is focused on recovery of such details for two quite distant examples: acclimation to extreme freezing tolerance in Siberian spruce (Picea obovata) and detection of proteins associated with prostate cancer.

The first biological system in the study, upon P. obovata, is interesting by this species ability to adapt and sustain extremely low temperatures, such as -60⁰C or below. Despite decades of investigations, the essential features and mechanisms of the amazing ability of this species still remains unclear. To enhance knowledge about extreme freezing tolerance, the metabolome and proteome of P. obovata’s needles were collected during the tree’s acclimation period, ranging from mid August to January, and have been analyzed.

The second system within this study is the plasma proteome analysis of high risk prostate cancer (PCa) patients, with and without bone metastases. PCa is one of the most common cancers among Swedish men, which can abruptly develop into an aggressive, lethal disease. The diagnostic tools, including PSA-tests, are insufficient in predicting the disease’s aggressiveness and novel prognostic markers are urgently required.

Both biological systems have been analyzed following similar steps: by two-dimensional difference gel electrophoresis (2D-DIGE) techniques, followed by protein identification using mass spectrometry (MS) analysis and multivariate methods. Data processing has been utilized for searching for proteins that serve as unique indicators for characterizing the status of the systems. In addition, the gas chromatography-mass spectrometry (GC-MS) study of the metabolic content of P.obovata’s needles, from the extended observation period, has been performed. The studies of both systems, combined with thorough statistical analysis of experimental outcomes, have resulted in novel insights and features for both P. obovata and prostate cancer. In particular, it has been shown that dehydrins, Hsp70s, AAA+ ATPases, lipocalin and several proteins involved in cellular metabolism etc., can be uniquely associated with acclimation to extreme freezing in conifers. Metabolomic analysis of P. obovata needles has revealed systematic metabolic changes in carbohydrate and lipid metabolism. Substantial increase of raffinose, accumulation of desaturated fatty acids, sugar acids, sugar alcohols, amino acids and polyamines that may act as compatible solutes or cryoprotectants have all been observed during the acclimation process.

Relevant proteins for prostate cancer progression and aggressiveness have been identified in the plasma proteome study, for patients with and without bone metastasis. Proteins associated with lipid transport, coagulation, inflammation and immune response have been found among them. 

Place, publisher, year, edition, pages
Umeå: Kemiska institutionen, Umeå Universitet, 2011. 44 p.
2D-DIGE, biomarkers, cold-acclimation, conifer, freezing tolerance, GC-MS, metabolomics, multiple hypothesis test, multivariate analysis, OPLS-DA, Picea obovata, plasma, prostate cancer, proteomics, ProteoMiner, Siberian spruce
National Category
Chemical Sciences
urn:nbn:se:umu:diva-43520 (URN)978-91-7459-204-7 (ISBN)
Public defence
2011-05-25, KBC-huset, KB3A9, Umeå Universitet, Umeå, 10:00 (English)
Available from: 2011-05-04 Created: 2011-05-03 Last updated: 2014-12-10Bibliographically approved

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