A plant-specific transcription factor IIB-related protein, pBRP2, is involved in endosperm growth control.
2011 (English)In: PloS one, ISSN 1932-6203, Vol. 6, no 2, e17216- p.Article in journal (Refereed) Published
General transcription factor IIB (TFIIB) and TFIIB-related factor (BRF), are conserved RNA polymerase II/III (RNAPII/III) selectivity factors that are involved in polymerase recruitment and transcription initiation in eukaryotes. Recent findings have shown that plants have evolved a third type of B-factor, plant-specific TFIIB-related protein 1 (pBRP1), which seems to be involved in RNAPI transcription. Here, we extend the repertoire of B-factors in plants by reporting the characterization of a novel TFIIB-related protein, plant-specific TFIIB-related protein 2 (pBRP2), which is found to date only in the Brassicacea family. Unlike other B-factors that are ubiquitously expressed, PBRP2 expression is restricted to reproductive organs and seeds as shown by RT-PCR, immunofluorescence labelling and GUS staining experiments. Interestingly, pbrp2 loss-of-function specifically affects the development of the syncytial endosperm, with both parental contributions required for wild-type development. pBRP2, is the first B-factor to exhibit cell-specific expression and regulation in eukaryotes, and might play a role in enforcing bi-parental reproduction in angiosperms.
Place, publisher, year, edition, pages
2011. Vol. 6, no 2, e17216- p.
IdentifiersURN: urn:nbn:se:umu:diva-43696DOI: 10.1371/journal.pone.0017216PubMedID: 21390310OAI: oai:DiVA.org:umu-43696DiVA: diva2:415252