Proteins that bind to misfolded mutant superoxide dismutase-1 in spinal cords from transgenic ALS model mice
2011 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 286, no 23, 20130-20136 p.Article in journal (Refereed) Published
Mutant superoxide dismutase-1 (SOD1) has an unidentified toxic property that provokes ALS. Several ALS-linked SOD1 mutations cause long C-terminal truncations, which suggests that common cytotoxic SOD1 conformational species should be misfolded and that the C-terminal end cannot be involved. The cytotoxicity may arise from interaction of cellular proteins with misfolded SOD1 species. Here we specifically immunocaptured misfolded SOD1 by the C-terminal end, from extracts of spinal cords from transgenic ALS model mice. Associated proteins were identified with proteomic techniques. Two transgenic models expressing SOD1s with contrasting molecular properties were examined: the stable G93A mutant, which is abundant in the spinal cord with only a tiny subfraction misfolded, and the scarce disordered truncation mutant G127insTGGG. For comparison, proteins in spinal cord extracts with affinity for immobilized apo G93A mutant SOD1 were determined. Two-dimensional gel patterns with a limited number of bound proteins were found, which were similar for the two SOD1 mutants. Apart from neurofilament light, the proteins identified were all chaperones and by far most abundant was Hsc70. The immobilized apo G93A SOD1, which would populate a variety of conformations, was found to bind to a considerable number of additional proteins. A substantial proportion of the misfolded SOD1 in the spinal cord extracts appeared to be chaperone-associated. Still, only about 1% of the Hsc70 appeared to be associated with misfolded SOD1. The results argue against the notion that chaperone depletion is involved in ALS pathogenesis in the transgenic models and in humans carrying SOD1 mutations.
Place, publisher, year, edition, pages
American Society for Biochemistry and Molecular Biology, 2011. Vol. 286, no 23, 20130-20136 p.
Amyotropic Lateral Sclerosis (Lou Gehrig's Disease), Heat Shock Protein, Neurodegeneration, Protein-Protein Interactions, Superoxide Dismutase (SOD), Transgenic Mice
Other Clinical Medicine
Research subject Clinical Chemistry
IdentifiersURN: urn:nbn:se:umu:diva-43895DOI: 10.1074/jbc.M111.218842PubMedID: 21493711ISBN: 1083-351X (Electronic) 0021-9258 (Linking)OAI: oai:DiVA.org:umu-43895DiVA: diva2:417136