msl2 mRNA is bound by free nuclear MSL complex in Drosophila melanogaster
2011 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 39, no 15, 6428-6439 p.Article in journal (Refereed) Published
In Drosophila, the global increase in transcription from the male X chromosome to compensate for its monosomy is mediated by the male-specific lethal (MSL) complex consisting of five proteins and two non-coding RNAs, roX1 and roX2. After an initial sequence-dependent recognition by the MSL complex of 150-300 high affinity sites, the spread to the majority of the X-linked genes depends on local MSL-complex concentration and active transcription. We have explored whether any additional RNA species are associated with the MSL complex. No additional roX RNA species were found, but a strong association was found between a spliced and poly-adenylated msl2 RNA and the MSL complex. Based on our results, we propose a model in which a non-chromatin-associated partial or complete MSL-complex titrates newly transcribed msl2 mRNA and thus regulates the amount of available MSL complex by feedback. This represents a novel mechanism in chromatin structure regulation.
Place, publisher, year, edition, pages
Oxford Journals , 2011. Vol. 39, no 15, 6428-6439 p.
Research subject Genetics
IdentifiersURN: urn:nbn:se:umu:diva-43980DOI: 10.1093/nar/gkr236PubMedID: 21551218OAI: oai:DiVA.org:umu-43980DiVA: diva2:417503
FunderSwedish Research Council