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G protein-coupled receptor mediated trimethylamine sensing
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
2009 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 25, no 4, 715-720 p.Article in journal (Refereed) Published
Abstract [en]

A new approach for the detection of trimethylamine (TMA) using a recombinant cell line of Xenopus laevis melanophores was developed. The cells were genetically modified to express the mouse trace amine-associated receptor 5 (mTAAR5), a G protein-coupled receptor from the mouse olfactory epithelium, which conferred high sensitivity to TMA. Cellular responses to TMA were analyzed by two different techniques, either by absorbance measurements using a microplate reader or by cellular imaging via an inverted microscope. A focused chemical screen allowed the discovery of additional, previously unknown stimuli of mTAAR5. The developed cell-based sensor demonstrated no sensitivity to trimethylamine N-oxide (TMAO), making it suitable for a straightforward evaluation of TMA levels in fish tissue extracts. For the detection of TMA vapor, the cells were covered with agarose, which allowed for intact cell viability for at least 6 h in air. The developed gas measurement platform was able to detect TMA from 1 to 100 ppm within 35 min.

Place, publisher, year, edition, pages
2009. Vol. 25, no 4, 715-720 p.
Keyword [en]
cell-based sensor, trace amine-associated receptor, TAAR5, trimethylamine detection, xenopus laevis melanophores
Identifiers
URN: urn:nbn:se:umu:diva-43987DOI: 10.1016/j.bios.2009.08.012PubMedID: 19734032OAI: oai:DiVA.org:umu-43987DiVA: diva2:417553
Available from: 2011-05-17 Created: 2011-05-17 Last updated: 2017-12-11Bibliographically approved

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