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Hydrogenase in Frankia KB5: Expression of and relation to nitrogenase
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-0536-903X
2000 (English)In: Canadian journal of microbiology (Print), ISSN 0008-4166, Vol. 46, no 12, 1091-1095 p.Article in journal (Refereed) Published
Abstract [en]

The localization and expression of the hydrogenase in free-living Frankia KB5 was investigated immunologically and by monitoring activity, focusing on its relationships with nitrogenase and H-2. Immunological studies revealed that the large subunit of the hydrogenase in Frankia KB5 was modified post-translationally, and transferred into the membrane after processing. The large subunit was constitutively expressed and no correlation was found between hydrogenase activity and synthesis. Although H-2 was not needed for induction of hydrogenase synthesis, exogenously added H-2 triggered hydrogen uptake in medium containing nitrogen, i.e., in the hyphae. A correlation between nitrogenase activity and hydrogen uptake was found in cultures grown in media without nitrogen, but interestingly the two enzymes showed no co-regulation.

Place, publisher, year, edition, pages
2000. Vol. 46, no 12, 1091-1095 p.
Keyword [en]
hydrogenase, expression, nitrogenase, Frankia KB5
URN: urn:nbn:se:umu:diva-44761ISI: 000165676800001OAI: diva2:424359
Available from: 2011-06-17 Created: 2011-06-09 Last updated: 2015-04-29

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Sellstedt, Anita
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Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
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