Hydrogenase in Frankia KB5: Expression of and relation to nitrogenase
2000 (English)In: Canadian journal of microbiology (Print), ISSN 0008-4166, Vol. 46, no 12, 1091-1095 p.Article in journal (Refereed) Published
The localization and expression of the hydrogenase in free-living Frankia KB5 was investigated immunologically and by monitoring activity, focusing on its relationships with nitrogenase and H-2. Immunological studies revealed that the large subunit of the hydrogenase in Frankia KB5 was modified post-translationally, and transferred into the membrane after processing. The large subunit was constitutively expressed and no correlation was found between hydrogenase activity and synthesis. Although H-2 was not needed for induction of hydrogenase synthesis, exogenously added H-2 triggered hydrogen uptake in medium containing nitrogen, i.e., in the hyphae. A correlation between nitrogenase activity and hydrogen uptake was found in cultures grown in media without nitrogen, but interestingly the two enzymes showed no co-regulation.
Place, publisher, year, edition, pages
2000. Vol. 46, no 12, 1091-1095 p.
hydrogenase, expression, nitrogenase, Frankia KB5
IdentifiersURN: urn:nbn:se:umu:diva-44761ISI: 000165676800001OAI: oai:DiVA.org:umu-44761DiVA: diva2:424359