umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Laser-based micromanipulation for separation and identification of individual Frankia vesicles
Show others and affiliations
2003 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 224, no 1, 97-100 p.Article in journal (Refereed) Published
Abstract [en]

In studies of symbiotic efficiency it is of great importance to identify and separate individual Frankia strains from a nodule. Therefore, a new laser-based micromanipulation technique has been developed in which individual vesicles from root nodules of two Frankia-Alnus symbioses have been successfully cut loose and separated from clusters of vesicles in sterile conditions under light microscopy using a laser scalpel and optical tweezers. Vesicles from the Alnus incana-Frankia AvCIl symbiosis were successfully isolated and grown in culture using this technique. The DNA from both Frankia sources was amplified by polymerase chain reaction (PCR). The work shows that a combination of laser-based manipulation techniques and PCR can be used for the separation and study of individual vesicles. This novel laser-based micromanipulation technique opens up various new possibilities, for instance, to study whether several Frankia strains can grow simultaneously in the same root nodule. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
2003. Vol. 224, no 1, 97-100 p.
Keyword [en]
DNA, laser scalpel, optical tweezers, PCR, RFLP, Frankia
Identifiers
URN: urn:nbn:se:umu:diva-44756DOI: 10.1016/S0378-1097(03)00435-XISI: 000184248400014OAI: oai:DiVA.org:umu-44756DiVA: diva2:424371
Available from: 2011-06-17 Created: 2011-06-09 Last updated: 2017-12-11

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Sellstedt, Anita

Search in DiVA

By author/editor
Sellstedt, Anita
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
FEMS Microbiology Letters

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 34 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf