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Expression of barley ADP-glucose pyrophosphorylase in Escherichia coli: processing and regulatory considerations
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). (Leszek Kleczkowski)ORCID iD: 0000-0001-8685-9665
1999 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 50, no 2, 209-214 p.Article in journal (Refereed) Published
Abstract [en]

Full length cDNAs for barley ADP-glucose pyrophosphorylase (AGPase) coding for the large subunits of the endosperm and leaf homologues of the enzyme (AGPase-S1 and -S2, respectively) and for the small subunit protein from endosperm (AGPase-B1), have been expressed in Escherichia coli. The cDNAs for AGPase-S1 and -S2 required different induction conditions for their maximal expression and they encoded immunologically distinct proteins. The AGPase-S1 that was produced by E. coli had the same M-r (58 kDa) as the corresponding protein in barley crude endosperm extracts, whereas the bacteria-produced AGPase-S2 (55 kDa) was larger than its counterpart from barley leaf preparations (53 kDa). An enzymatically active ACPase expressed in E. coli from a double construct containing cDNAs for AGPase-S1 and -B1 subunits was insensitive to the activation by 3-phosphoglycerate and to inhibition by inorganic phosphate, similarly to the enzyme in barley endosperm. Neither AGPase-S1 nor -B1 were active when expressed alone in the bacteria. The data are discussed with respect to possible mechanisms of intracellular targeting of immature AGPase-S proteins in barley tissues and regarding previous data on effector regulation of the barley enzyme. (C) 1998 Elsevier Science Ltd. All rights reserved.

Place, publisher, year, edition, pages
1999. Vol. 50, no 2, 209-214 p.
Keyword [en]
Hordeum vulgare, Gramineae, Poaceae, barley, starch synthesis, Brittle-2, Shrunken-2, heterologous expression, transit peptide
URN: urn:nbn:se:umu:diva-44743ISI: 000078216500003OAI: diva2:426349
Available from: 2011-06-23 Created: 2011-06-09 Last updated: 2015-04-29

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