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Glucose and mannose regulate the expression of a major sucrose synthase gene in Arabidopsis via hexokinase-dependent mechanisms
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). (Leszek Kleczkowski)ORCID iD: 0000-0001-8685-9665
2002 (English)In: Plant physiology and biochemistry (Paris), ISSN 0981-9428, Vol. 40, no 11, 907-911 p.Article in journal (Refereed) Published
Abstract [en]

Sucrose synthase (SuSy) is an important enzyme involved in sucrose synthesis/breakdown in all plants. Sus1, a major SuSy gene in Arabidopsis thaliana, was upregulated by sucrose, glucose and D-mannose, but not 3-O-methylglucose, when those compounds were fed to excised leaves. Mannos, was more effective than glucose or sucrose in the induction of Sus1, with strong effects observed at a concentration as low as 20, mM. When fed to the excised leaves, N-acetyl-glucosamine, an inhibitor of hexokinase (HXK) enzymatic activity, decreased sucrose- and glucose-dependent, but not mannose-dependent, upregulation of Sus1. The sucrose/glucose-dependent Sus1 expression was strongly induced in transgenic Arabidopsis HXK-overexpressing (OE) plants, whereas mannose-dependent Sus1 expression markedly decreased in OE, but not in HXK-"antisense", Arabidopsis plants. Feeding with sucrose resulted in a marked increase of glucose content in leaves, suggesting that it is glucose rather than sucrose that serves as a signal in upregulating Sus1 expression in sucrose-fed plants. The data suggest that Sus1 is regulated by a HXK-dependent pathway, with glucose and mannose effects differentially sensed/transmitted via the HXK step. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

Place, publisher, year, edition, pages
2002. Vol. 40, no 11, 907-911 p.
Keyword [en]
Arabidopsis thaliana, gene expression, sucrose synthesis, Sus1, UDP-glucose synthesis
URN: urn:nbn:se:umu:diva-44729ISI: 000179850800002OAI: diva2:426366
Available from: 2011-06-23 Created: 2011-06-09 Last updated: 2015-04-29

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