Change search
ReferencesLink to record
Permanent link

Direct link
The small subunit ADP-glucose pyrophosphorylase (ApS) promoter mediates okadaic acid-sensitive uidA expression in starch-synthesizing tissues and cells in Arabidopsis
Show others and affiliations
2003 (English)In: Planta, ISSN 0032-0935, E-ISSN 1432-2048, Vol. 217, no 2, 184-192 p.Article in journal (Refereed) Published
Abstract [en]

Transgenic plants of Arabidopsis thaliana Heynh., transformed with a bacterial beta-glucuronidase (GUS) gene under the control of the promoter of the small subunit (ApS) of ADP-glucose pyrophosphorylase (AGPase), exhibited GUS staining in leaves (including stomata), stems, roots and flowers. Cross-sections of stems revealed GUS staining in protoxylem parenchyma, primary phloem and cortex. In young roots, the staining was found in the root tips, including the root cap, and in vascular tissue, while the older root-hypocotyl axis showed prominent staining in the secondary phloem and paratracheary parenchyma of secondary xylem. The GUS staining co-localized with ApS protein, as found by tissue printing using antibodies against ApS. Starch was found only in cell and tissue types exhibiting GUS staining and ApS labelling, but not in all of them. For example, starch was lacking in the xylem parenchyma and secondary phloem of the root-hypocotyl axis. Sucrose potently activated ApS gene expression in leaves of wild-type (wt) plants, and in transgenic seedlings grown on sucrose medium where GUS activity was quantified with 4-methylumbelliferyl-beta-glucuronide as substrate. Okadaic acid, an inhibitor of protein phosphatases 1 and 2A, completely blocked expression of ApS in mature leaves of wt plants and prevented GUS staining in root tips and flowers of the transgenic plants, suggesting a similar signal transduction mechanism for ApS expression in various tissues. The data support the key role of AGPase in starch synthesis, but they also underlie the ubiquitous importance of the ApS gene for AGPase function in all organs/tissues of Arabidopsis.

Place, publisher, year, edition, pages
2003. Vol. 217, no 2, 184-192 p.
Keyword [en]
ADP-glucose pyrophosphorylase, Arabidopsis, GUS expression, starch synthesis, sucrose
URN: urn:nbn:se:umu:diva-44728DOI: 10.1007/s00425-003-0982-yISI: 000183451700002OAI: diva2:426367
Available from: 2011-06-23 Created: 2011-06-09 Last updated: 2015-04-29

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Kleczkowski, Leszek A
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 11 hits
ReferencesLink to record
Permanent link

Direct link