Change search
ReferencesLink to record
Permanent link

Direct link
Cloning of the cpce and cpcf genes from synechococcus sp pcc-6301 and their inactivation in synechococcus sp pcc-7942
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-2147-7428
1994 (English)In: Plant Molecular Biology, ISSN 0167-4412, E-ISSN 1573-5028, Vol. 26, no 1, 313-326 p.Article in journal (Refereed) Published
Abstract [en]

Two open reading frames denoted as cpcE and cpcF were cloned and sequenced from Synechococcus sp. PCC 6301. The cpcE and cpcF genes are located downstream of the cpcB2A2 gene cluster in the phycobilisome rod operon and can be transcribed independently of the upstream cpcB2A2 gene cluster. The cpcE and cpcF genes were separately inactivated by insertion of a kanamycin resistance cassette in Synechococcus sp. PCC 7942 to generate mutants R2EKM and R2FKM, respectively, both of which display a substantial reduction in spectroscopically detectable phycocyanin. The levels of beta- and alpha-phycocyanin polypeptides were reduced in the R2EKM and R2FKM mutants although the phycocyanin and linker genes are transcribed at normal levels in the mutants as in the wild type indicating the requirement of the functional cpcE and cpcF genes for normal accumulation of phycocyanin. Two biliprotein fractions were isolated on sucrose density gradient from the R2EKM/R2FKM mutants. The faster sedimenting fraction consisted of intact phycobilisomes. The slower sedimenting biliprotein fraction was found to lack phycocyanin polypeptides, thus no free phycocyanin was detected in the mutants. Characterization of the phycocyanin from the mutants revealed that it was chromophorylated, had a lambda(max) similar to that from the wild type and could be assembled into the phycobilisome rods. Thus, although phycocyanin levels are reduced in the R2EKM and R2FKM mutants, the remaining phycocyanin seems to be chromophorylated and similar to that in the wild type with respect to phycobilisome rod assembly and energy transfer to the core.

Place, publisher, year, edition, pages
1994. Vol. 26, no 1, 313-326 p.
Keyword [en]
synechococcus 6301, synechococcus 7942, phycocyanin, CPCE, CPCF, gene activation
URN: urn:nbn:se:umu:diva-44723ISI: A1994PQ58900030OAI: diva2:426389
Available from: 2011-06-23 Created: 2011-06-09 Last updated: 2016-02-29

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Gustafsson, Petter
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
Plant Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 35 hits
ReferencesLink to record
Permanent link

Direct link