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Cloning and sequencing of human kappa-casein cDNA.
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Paediatrics.
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1992 (English)In: DNA Sequence, ISSN 1042-5179, E-ISSN 1029-2365, Vol. 3, no 4, 245-6 p.Article in journal (Refereed) Published
Abstract [en]

A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.

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1992. Vol. 3, no 4, 245-6 p.
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URN: urn:nbn:se:umu:diva-45444PubMedID: 1296818OAI: oai:DiVA.org:umu-45444DiVA: diva2:429458
Available from: 2011-07-04 Created: 2011-07-04 Last updated: 2017-12-11

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Hernell, Olle

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