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McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR
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2009 (English)In: Science, ISSN 0036-8075, Vol. 324, no 5932, 1323-1327 p.Article in journal (Refereed) Published
Abstract [en]

All living organisms face a variety of environmental stresses that cause the misfolding and aggregation of proteins. To eliminate damaged proteins, cells developed highly efficient stress response and protein quality control systems. We performed a biochemical and structural analysis of the bacterial CtsR/McsB stress response. The crystal structure of the CtsR repressor, in complex with DNA, pinpointed key residues important for high-affinity binding to the promoter regions of heat-shock genes. Moreover, biochemical characterization of McsB revealed that McsB specifically phosphorylates arginine residues in the DNA binding domain of CtsR, thereby impairing its function as a repressor of stress response genes. Identification of the CtsR/McsB arginine phospho-switch expands the repertoire of possible protein modifications involved in prokaryotic and eukaryotic transcriptional regulation.

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American Association for the Advancement of Science , 2009. Vol. 324, no 5932, 1323-1327 p.
URN: urn:nbn:se:umu:diva-45508DOI: 10.1126/science.1170088PubMedID: 19498169OAI: diva2:430074
Available from: 2011-07-06 Created: 2011-07-06 Last updated: 2011-08-24Bibliographically approved

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Charpentier, Emmanuelle
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Department of Molecular Biology (Faculty of Medicine)Umeå Centre for Microbial Research (UCMR)Molecular Infection Medicine Sweden (MIMS)
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