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Characterization of a new class of tetracycline-resistance gene tet(S) in Listeria monocytogenes BM4210.
1993 (English)In: Gene, ISSN 0378-1119, E-ISSN 1879-0038, Vol. 131, no 1, p. 27-34Article in journal (Refereed) Published
Abstract [en]

The nucleotide sequence of the tetracycline (Tc)-minocycline (Mc)-resistance determinant of plasmid pIP811 from Listeria monocytogenes BM4210 has been determined. The gene, designated tet(S), was identified by analysis of the start and stop codons as a coding sequence of 1923 bp, corresponding to a protein with a calculated M(r) of 72,912. The apparent 68-kDa size estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the protein characterized in a cell-free coupled transcription-translation system was in good agreement with the calculated value. The tet(S) gene product exhibits 79 and 72% amino acid identity with Tet(M) from Streptococcus pneumoniae and Tet(O) from Campylobacter coli, respectively. The distribution of tet(S) in strains of Gram+ and Gram- genera resistant to Tc (TcR) and Mc (McR) was studied by hybridization under high stringency using a 590-bp intragenic probe. Homology with tet(S) was detected in two clinical isolates of L. monocytogenes isolated in different geographical areas.

Place, publisher, year, edition, pages
1993. Vol. 131, no 1, p. 27-34
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URN: urn:nbn:se:umu:diva-45536PubMedID: 8370538OAI: oai:DiVA.org:umu-45536DiVA, id: diva2:430104
Available from: 2011-07-07 Created: 2011-07-07 Last updated: 2018-06-08

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Charpentier, E

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