umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A common telomeric gene silencing assay is affected by nucleotide metabolism
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. (Chabes)
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). (Chabes)ORCID iD: 0000-0003-1708-8259
Show others and affiliations
2011 (English)In: Molecular Cell, ISSN 1097-2765, E-ISSN 1097-4164, Vol. 42, no 1, 127-136 p.Article in journal (Refereed) Published
Abstract [en]

Telomere-associated position-effect variegation (TPEV) in budding yeast has been used as a model for understanding epigenetic inheritance and gene silencing. A widely used assay to identify mutants with improper TPEV employs the URA3 gene at the telomere of chromosome VII-L that can be counterselected with 5-fluoroorotic acid (5-FOA). 5-FOA resistance has been inferred to represent lack of transcription of URA3 and therefore to represent heterochromatin-induced gene silencing. For two genes implicated in telomere silencing, POL30 and DOT1, we show that the URA3 telomere reporter assay does not reflect their role in heterochromatin formation. Rather, an imbalance in ribonucleotide reductase (RNR), which is induced by 5-FOA, and the specific promoter of URA3 fused to ADH4 at telomere VII-L are jointly responsible for the variegated phenotype. We conclude that metabolic changes caused by the drug employed and certain mutants being studied are incompatible with the use of certain prototrophic markers for TPEV.

Place, publisher, year, edition, pages
Elsevier (Cell Press) , 2011. Vol. 42, no 1, 127-136 p.
Identifiers
URN: urn:nbn:se:umu:diva-45631DOI: 10.1016/j.molcel.2011.03.007PubMedID: 21474074OAI: oai:DiVA.org:umu-45631DiVA: diva2:432773
Available from: 2011-08-05 Created: 2011-08-05 Last updated: 2017-12-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Tsaponina, OlgaChabes, Andrei
By organisation
Department of Medical Biochemistry and BiophysicsMolecular Infection Medicine Sweden (MIMS)
In the same journal
Molecular Cell

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 99 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf