umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Altering flux through the sucrose biosynthesis pathway in transgenic Arabidopsis thaliana modifies photosynthetic acclimation at low temperatures and the development of freezing tolerance
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0001-6664-0471
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-2147-7428
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0001-5900-7395
Show others and affiliations
2003 (English)In: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040, Vol. 26, no 4, 523-535 p.Article in journal (Refereed) Published
Abstract [en]

To test the hypothesis that the up-regulation of sucrose biosynthesis during cold acclimation is essential for the development of freezing tolerance, the acclimation responses of wild-type (WT) Arabidopsis thaliana (Heynh.) were compared with transgenic plants over-expressing sucrose phosphate synthase (over-sps) or with antisense repression of either cytosolic fructose-1,6-bisphosphatase (antifbp) or sucrose phosphate synthase (antisps). Plants were grown at 23 degreesC and then shifted to 5 degreesC. The leaves shifted to 5 degreesC for 10 d and the new leaves that developed at 5 degreesC were compared with control leaves on plants at 23 degreesC. Plants over-expressing sucrose phosphate synthase showed improved photosynthesis and increased flux of fixed carbon into sucrose when shifted to 5 degreesC, whereas both antisense lines showed reduced flux into soluble sugars relative to WT. The improved photosynthetic performance by the over-sps plants shifted to 5 degreesC was associated with an increase in freezing tolerance relative to WT (-9.1 and -7.2 degreesC, respectively). In contrast, both antisense lines showed impaired development of freezing tolerance (- 5.2 and -5.8 degreesC for antifbp and antisps, respectively) when shifted to 5 degreesC. In the new leaves developed at 5 degreesC the recovery of photosynthesis as typically seen in WT was strongly inhibited in both antisense lines and this inhibition was associated with a further failure of both antisense lines to cold acclimate. Thus, functional sucrose biosynthesis at low temperature in the over-sps plants reduced the inhibition of photosynthesis, maintained the mobilization of carbohydrates from source leaves to sinks and increased the rate at which freezing tolerance developed. Modification of sucrose metabolism therefore represents an additional approach that will have benefits both for the development of freezing tolerance and over-wintering, and for the supply of exportable carbohydrate to support growth at low temperatures.

Place, publisher, year, edition, pages
2003. Vol. 26, no 4, 523-535 p.
Keyword [en]
Arabidopsis, carbon metabolism, cold acclimation, photosynthesis, sucrose phosphate synthase, sucrose
Identifiers
URN: urn:nbn:se:umu:diva-44652ISI: 000182009600005OAI: oai:DiVA.org:umu-44652DiVA: diva2:434122
Available from: 2011-08-12 Created: 2011-06-09 Last updated: 2017-12-08

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Strand, ÅsaGustafsson, PetterGardeström, PerHurry, Vaughan
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
Plant, Cell and Environment

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 96 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf