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ISOLATION, PURIFICATION, AND CHARACTERIZATION OF MITOCHONDRIA FROM CHLAMYDOMONAS-REINHARDTII
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0001-5900-7395
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0003-2133-6526
1995 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 107, no 2, 479-483 p.Article in journal (Refereed) Published
Abstract [en]

Mitochondria were isolated from autotrophically grown Chlamydomonas reinhardtii cell-wall-less mutant CW 92. The cells were broken by vortexing with glass beads, and the mitochondria were collected by differential centrifugation and purified on a Percoll gradient. The isolated mitochondria oxidized malate, pyruvate, succinate, NADH, and a-ketoglutarate. Respiratory control was obtained with malate (2.0) and pyruvate (2.2) but not with the other substrates. From experiments with KCN and salicylhydroxamic acid, it was estimated that the capacity of the cytochrome pathway was at least 100 nmol O-2 mg(-1) protein min(-1) and the capacity of the alternative oxidase was at least 50 nmol O-2 mg(-1) protein min(-1). A low sensitivity to oligomycin indicates some difference in the properties of the mitochondrial ATPase from Chlamydomonas as compared to higher plants.

Place, publisher, year, edition, pages
1995. Vol. 107, no 2, 479-483 p.
Identifiers
URN: urn:nbn:se:umu:diva-44644ISI: A1995QH87200020OAI: oai:DiVA.org:umu-44644DiVA: diva2:434132
Available from: 2011-08-12 Created: 2011-06-09 Last updated: 2017-12-08

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Eriksson, MatsGardeström, PerSamuelsson, Göran

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