umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A carbonic anhydrase catalyzed CO2 pump in Chlamydomonas reinhardtii: In vivo experiments, computer modelling, and theory
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-6002-929X
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Show others and affiliations
2000 (English)In: Russian journal of plant physiology, ISSN 1021-4437, E-ISSN 1608-3407, Vol. 47, no 5, 613-621 p.Article in journal (Refereed) Published
Abstract [en]

We studied the bicarbonate depletion effects on photosynthesis in high-CO2 grown-cell wall-less mutants of Chlamydomonas reinhardtii with (cw92) and without (cia3/cw15) active carbonic anhydrase (CA) associated with photosystem II (PSII). The mutants were grown in a high-CO2 medium and then washed with a CO2-free buffer. We discuss the shutdown of oxygen evolution in cw92 as a consequence of CO2 depletion of the Calvin cycle. It is suggested that CA acts as part of a PSII-driven pump delivering CO2 from the lumen into the stroma, where it becomes available to the Calvin cycle. Our model is supported by computer simulation data and accommodates the results of in vivo experiments as well as the differences between cw92 and cia3 mutants. We also present a simple theory of the phenomena discussed, based on a time-scale hierarchy approach, which describes the CO2 depletion in cw92 using only two rate constants-V-max and K-m of the Calvin cycle.

Place, publisher, year, edition, pages
2000. Vol. 47, no 5, 613-621 p.
Keyword [en]
carbonic anhydrase, thylakoid, carbon metabolism, mathematical modelling
Identifiers
URN: urn:nbn:se:umu:diva-44626ISI: 000089482700007OAI: oai:DiVA.org:umu-44626DiVA: diva2:434817
Available from: 2011-08-16 Created: 2011-06-09 Last updated: 2017-12-08

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Karlsson, JanOquist, GunnarSamuelsson, Göran
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
Russian journal of plant physiology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 79 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf