Characterization of photosystem II antenna complexes separated by non-denaturing isoelectric focusing
1998 (English)In: Zeitschrift für Naturforschung C - A Journal of Biosciences, ISSN 0939-5075, Vol. 53, no 9-10, 841-848 p.Article in journal (Refereed) Published
CP26, CP29 and three different LHC II subcomplexes have been purified from a carnation photosystem II (PSII) preparation using non-denaturing isoelectric focusing in a vertical polyacrylamide slab gel. The identity of the fractions was established by absorption spectroscopy, SDS-PAGE and immunoblotting. CP26 comprised a single apoprotein of 26.6 kDa and CP29 contained two apoproteins of 28.8 and 28.5 kDa. LHC II subcomplex A consisted of Lhcb1 homotrimers, and subcomplexes B and C consisted of Lhcb1/Lhcb2 and Lhcb1/Lhcb3 heterotrimers, respectively. We discuss the data in relation to the organization of the PS II antenna in vivo.
Place, publisher, year, edition, pages
1998. Vol. 53, no 9-10, 841-848 p.
chlorophyll alpha/beta, protein, light-harvesting, polypeptide, subcomplex, trimer
IdentifiersURN: urn:nbn:se:umu:diva-44609ISI: 000076790000010OAI: oai:DiVA.org:umu-44609DiVA: diva2:434836