Phosphorylated CpxR Restricts Production of the RovA Global Regulator in Yersinia pseudotuberculosis
2011 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 6, no 8, e23314- p.Article in journal (Refereed) Published
Background: RovA is a global transcriptional regulator of gene expression in pathogenic Yersinia. RovA levels are kept in check by a sophisticated layering of distinct transcriptional and post-transcriptional regulatory mechanisms. In the enteropathogen Y. pseudotuberculosis, we have previously reported that the extracytoplasmic stress sensing CpxA-CpxR two-component regulatory system modulates rovA expression.
Methodology/Principal Findings: In this study, we characterized CpxR phosphorylation (CpxR similar to P) in vitro, and determined that phosphorylation was necessary for CpxR to efficiently bind to the PCR-amplified upstream regulatory region of rovA. The precise CpxR similar to P binding site was mapped by a nuclease protection assay and directed mutagenesis confirmed that in vivo binding to the rovA promoter inhibits transcription. Reduced RovA production was most pronounced following CpxR, P accumulation in the Yersinia cytoplasm during chronic Cpx pathway activation and by the indiscriminate phosphodonor action of acetyl phosphate.
Conclusions/Significance: Cpx pathway activation restricts levels of the RovA global regulator. The regulatory influence of CpxR similar to P must therefore extend well beyond periplasmic quality control in the Yersinia envelope, to include genes involved in environmental survival and pathogenicity.
Place, publisher, year, edition, pages
Public Library of Science , 2011. Vol. 6, no 8, e23314- p.
IdentifiersURN: urn:nbn:se:umu:diva-46172DOI: 10.1371/journal.pone.0023314OAI: oai:DiVA.org:umu-46172DiVA: diva2:437258
FunderSwedish Research CouncilKnut and Alice Wallenberg Foundation