umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Multiplex PCR as a tool for validating plasmid content of Borrelia burgdorferi.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Bergström)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Bergström)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Bergström)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Bergström)
2011 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 86, no 2, 243-7 p.Article in journal (Refereed) Published
Abstract [en]

Borrelia burgdorferi has an unusual genomic structure containing 21 plasmids. These plasmids carry genes that are essential for infectivity and survival of the spirochetes in vivo. Several plasmids are lost during cultivation in vitro, which might lead to a heterogeneous population after multiple passages and loss of infectivity in laboratory animals. Herein, we present a simple and inexpensive multiplex PCR method that detects the complete plasmid profile of B. burgdorferi B31 in just two PCR tubes.

Place, publisher, year, edition, pages
2011. Vol. 86, no 2, 243-7 p.
Identifiers
URN: urn:nbn:se:umu:diva-46238DOI: 10.1016/j.mimet.2011.05.004PubMedID: 21605603OAI: oai:DiVA.org:umu-46238DiVA: diva2:437479
Available from: 2011-08-29 Created: 2011-08-29 Last updated: 2017-12-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Bunikis, IgnasBonde, MariKutschan-Bunikis, SabrinaBergström, Sven

Search in DiVA

By author/editor
Bunikis, IgnasBonde, MariKutschan-Bunikis, SabrinaBergström, Sven
By organisation
Department of Molecular Biology (Faculty of Medicine)
In the same journal
Journal of Microbiological Methods

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 148 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf