Change search
ReferencesLink to record
Permanent link

Direct link
Polycomb silencing blocks transcription initiation.
University of Geneva, Switzerlandc.
Show others and affiliations
2004 (English)In: Molecular Cell, ISSN 1097-2765, E-ISSN 1097-4164, Vol. 13, no 6, 887-93 p.Article in journal (Refereed) Published
Abstract [en]

Polycomb (PcG) complexes maintain the silent state of target genes. The mechanism of silencing is not known but has been inferred to involve chromatin packaging to block the access of transcription factors. We have studied the effect of PcG silencing on the hsp26 heat shock promoter. While silencing does decrease the accessibility of some restriction enzyme sites to some extent, it does not prevent the binding of TBP, RNA polymerase, or the heat shock factor to the hsp26 promoter, as shown by chromatin immunoprecipitation. However, we find that in the repressed state, the RNA polymerase cannot initiate transcription. We conclude that, rather than altering chromatin structure to block accessibility, PcG silencing in this construct targets directly the activity of the transcriptional machinery at the promoter.

Place, publisher, year, edition, pages
2004. Vol. 13, no 6, 887-93 p.
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:umu:diva-46243PubMedID: 15053881OAI: diva2:437492
Available from: 2011-08-29 Created: 2011-08-29 Last updated: 2011-09-21Bibliographically approved

Open Access in DiVA

No full text

Other links


Search in DiVA

By author/editor
Schwartz, Yuri B
In the same journal
Molecular Cell
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 25 hits
ReferencesLink to record
Permanent link

Direct link