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Attachment of HeT-A sequences to chromosomal termini in Drosophila melanogaster may occur by different mechanisms
Institute of Gene Biology, RAS, Russia.
Institute of Gene Biology, RAS, Russia.
Institute of Gene Biology, RAS, Russia.
2000 (English)In: Molecular and Cellular Biology, ISSN 0270-7306, E-ISSN 1098-5549, Vol. 20, no 20, 7634-7642 p.Article in journal (Refereed) Published
Abstract [en]

Drosophila telomeres contain arrays of the retrotransposonlike elements HeT-A and TART. Their transposition to broken chromosomal termini has been implicated in chromosome healing and telomere elongation. The HeT-A element is attached by its 3' end, which contains the promoter. To monitor the behavior of HeT-A elements, we used the yellow gene with terminal deficiencies consisting of breaks in the yellow promoter region that result in the y-null phenotype. Attachment of the HeT-A element provides the promoterless yellow gene with a promoter that activates yellow expression in bristles. The frequency of HeT-A transpositions to the yellow terminal deficiency depends on the genotype of the line and varies from 2 x 10(-3) to less than 2 x 10(-5). Loss of the attached HeT-A due to incomplete replication at the telomere leads to inactivation of yellow expression, which is restored by attachment of a new HeT-A element upstream of yellow. New HeT-A additions occur at a frequency of about 1.2 x 10(-3). Short DNA attachments are generated by gene conversion using the homologous telomeric sequences as templates. Longer DNA attachments are generated either by conventional transposition of an HeT-A element to the chromosomal terminus or by recombination between the 3' terminus of telomeric HeT-A elements and the receding end of HeT-A attached to the yellow gene.

Place, publisher, year, edition, pages
2000. Vol. 20, no 20, 7634-7642 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-46250DOI: 0270-7306/00/$04.0010PubMedID: 11003659OAI: oai:DiVA.org:umu-46250DiVA: diva2:437504
Available from: 2011-08-29 Created: 2011-08-29 Last updated: 2017-12-08Bibliographically approved

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Kahn, Tatiana

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