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Analyzing P-bodies in Saccharomyces cerevisiae
The University of Arizona, Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, Tucson, Arizona, USA.
2008 (English)In: Methods in Enzymology, ISSN 0076-6879, E-ISSN 1557-7988, ISSN 1557-7988, Vol. 448, 507-520 p.Article in journal (Refereed) Published
Abstract [en]

Cytoplasmic processing bodies, or P-bodies, are RNA-protein granules found in eukaryotic cells. P-bodies contain non-translating mRNAs and proteins involved in mRNA degradation and translational repression. P-bodies, and the mRNPs within them, have been implicated in mRNA storage, mRNA degradation, and translational repression. The analysis of mRNA turnover often involves the analysis of P-bodies. In this chapter, we describe methods to analyze P-bodies in the budding yeast, Saccharomyces cerevisiae, including procedures to determine whether a protein or mRNA can accumulate in P-bodies, whether an environmental perturbation or mutation affects P-body size and number, and methods to quantify P-bodies.

Place, publisher, year, edition, pages
2008. Vol. 448, 507-520 p.
Keyword [en]
cytoplasmic processing bodies messenger-rna decay translational repression human-cells gene-expression global analysis body formation budding yeast localization stability
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-46251DOI: 10.1016/S0076-6879(08)02625-6PubMedID: 19111192OAI: oai:DiVA.org:umu-46251DiVA: diva2:437506
Available from: 2011-08-29 Created: 2011-08-29 Last updated: 2017-12-08Bibliographically approved

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