Change search
ReferencesLink to record
Permanent link

Direct link
60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm
Biochemie-Zentrum Heidelberg, Universität Heidelberg, Germany.
Show others and affiliations
2002 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 21, no 20, 5539-5547 p.Article in journal (Refereed) Published
Abstract [en]

60S ribosomes undergo initial assembly in the nucleolus before export to the cytoplasm and recent analyses have identified several nucleolar pre-60S particles. To unravel the steps in the pathway of ribosome formation, we have purified the pre-60S ribosomes associated with proteins predicted to act at different stages as the pre-ribosomes transit from the nucleolus through the nucleoplasm and are then exported to the cytoplasm for final maturation. About 50 non-ribosomal proteins are associated with the early nucleolar pre-60S ribosomes. During subsequent maturation and transport to the nucleoplasm, many of these factors are removed, while others remain attached and additional factors transiently associate. When the 60S precursor particles are close to exit from the nucleus they associate with at least two export factors, Nmd3 and Mtr2. As the 60S pre-ribosome reaches the cytoplasm, almost all of the factors are dissociated. These data provide an initial biochemical map of 60S ribosomal subunit formation on its path from the nucleolus to the cytoplasm.

Place, publisher, year, edition, pages
2002. Vol. 21, no 20, 5539-5547 p.
Keyword [en]
Nmd3, nuclear export, nucleolus, rRNA processing, 60S subunit
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:umu:diva-46254DOI: 10.1093/emboj/cdf547PubMedID: 12374754OAI: diva2:437509
Available from: 2011-08-29 Created: 2011-08-29 Last updated: 2011-09-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Nissan, Tracy A
In the same journal
EMBO Journal
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 41 hits
ReferencesLink to record
Permanent link

Direct link